Molecular analysis of Toxoplasma gondii Surface Antigen 1 (SAG1) gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis
نویسندگان
چکیده
منابع مشابه
Cloning and sequencing of Toxoplasma gondii major surface antigen (SAG1) gene
Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...
متن کاملThe SAG1 Toxoplasma gondii surface protein is not required for acute ocular toxoplasmosis in mice.
The SAG1 Toxoplasma gondii surface protein stimulates acute ileitis. To determine whether SAG1 is also important in the eye, wild-type or SAG1 knockout parasites were injected intravitreally into mice. No differences in retinal damage or parasite growth were observed, indicating that unlike the case for the intestine, factors besides SAG1 are important for retinal damage.
متن کاملAnalysis of Toxoplasma gondii antigens with sera from toxoplasmosis patients.
Some proteins of the Toxoplasma gondii are recognized by IgG, IgM and IgA antibodies in patients with acute and chronic toxoplasmosis, depending on the strain and stage of the Toxoplasma. Sixty-nine sera from immunocompetent individuals were studied through the Western-Blot Test: 20 has an acute infection, 29 has a chronic toxoplasmosis infection and 20 were healthy (seronegatives). The protein...
متن کاملCloning of Major Surface Antigen (SAG1) Gene of Toxoplasma gondii Tachyzoites
Toxoplasma gondii is an obligate intracellular protozoon parasite of all the warm blooded animals including birds and humans. It completes a sexual part of its life cycle in the intestinal epithelial cell of the Felidae (definitive host) and an asexual part in warm blooded (intermediate host). Human is an accidental host who contracts infection through handling cat litter and gardening, ingesti...
متن کاملToxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding.
Previous studies have indicated that SAG1, the major surface molecule of the protozoan parasite Toxoplasma gondii, is an important attachment ligand for the host cell. However, the research data that supports this claim comes largely from studies investigating tachyzoite binding, and not SAG1 binding per se. In this study we successfully developed an in vitro attachment assay to directly evalua...
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ژورنال
عنوان ژورنال: Journal of Physics: Conference Series
سال: 2018
ISSN: 1742-6588,1742-6596
DOI: 10.1088/1742-6596/1022/1/012048